Oleg V. Krokhin, Ph.D.

Assistant Professor: Department of Internal Medicine, University of Manitoba: Jan 2007 - present

Adjunct Professor: Department of Chemistry, University of Manitoba : 2009 - present

Senior Scientist: Manitoba Centre for Proteomics and Systems Biology, University of Manitoba: May 2006 - present

Research Associate: Department of Internal Medicine, Faculty of Medicine, Manitoba Centre for Proteomics and Systems Biology, University of Manitoba: Sept 2005 - May 2006

Research Associate: Department of Physics and Astronomy, University of Manitoba, Time-of-Flight Laboratory: Oct 2000 - Sept 2005

Senior Scientist, Chromatography Laboratory: Moscow State University, Moscow, Russia: 1997 - 2000

Group Leader, Capillary Electrophoresis: Moscow State University, Moscow, Russia: 1995 - 2000

Scientist, Chromatography Laboratory: Moscow State University, Moscow, Russia: 1994-1997

Junior Scientist, Chromatography Laboratory: Moscow State University, Moscow, Russia: 1992-1994


LEARN ABOUT OUR WORLD-LEADING APPROACH TO CALCULATE PEPTIDE'S HYDROPHOBISITY IN REVERSED-PHASE HPLC: Sequence Specific Retention Calculator (SSRCalc)


EDUCATION:

Doctor of Philosophy, Chemistry 1993 Laboratory of Chromatography, Chemistry Department, Moscow State University, Moscow, Russia (Prof. O.A. Shpigun)

Bachelor of Science, Chemistry 1989 Laboratory of Preconcentration methods, Chemistry Department, Moscow State University, Moscow, Russia. (Prof. O. N. Obrezkov)


MAIN FIELDS OF SCIENTIFIC INTEREST:

application of bio-separation techniques for protein analysis, proteomics, bioinformatics, mass-spectrometry, protein/peptide chemistry, HPLC of peptides: retention prediction algorithms, quantitative protein analysis, wide range of separation chemistry applications (HPLC, capillary electrophoresis).

Selected Publications (Proteomics and RP-HPLC fundamental studies):

1. Vu, H.; Spicer, V.; Gotfrid, A.; Krokhin, O. V., A model for predicting slopes S in the basic equation for the linear-solvent-strength theory of peptide separation by reversed-phase high-performance liquid chromatography. J Chromatogr A 2010, 1217, (4), 489-97.

2. McCubbin, J. A.; Hosseini, H.; Krokhin, O. V., Boronic Acid catalyzed friedel-crafts reactions of allylic alcohols with electron-rich arenes and heteroarenes. J Org Chem 2010, 75, (3), 959-62.

3. Ghosh, D.; Lippert, D.; Krokhin, O.; Cortens, J. P.; Wilkins, J. A., Defining the membrane proteome of NK cells. J Mass Spectrom 2010, 45, (1), 1-25.

4. Dwivedi, R. C.; Krokhin, O. V.; Cortens, J. P.; Wilkins, J. A., Assessment of the reproducibility of random hexapeptide peptide library-based protein normalization. J Proteome Res 2010, 9, (2), 1144-9.

5. Andreev, V. P.; Dwivedi, R. C.; Paz-Filho, G.; Krokhin, O. V.; Wong, M. L.; Wilkins, J. A.; Licinio, J., Dynamics of plasma proteome during leptin-replacement therapy in genetically based leptin deficiency. Pharmacogenomics J. 2010, in press.

6. Krokhin, O. V.; Spicer, V., Peptide retention standards and hydrophobicity indexes in reversed-phase high-performance liquid chromatography of peptides. Anal Chem 2009, 81, (22), 9522-30.

7. Ho, J.; Lucy, M.; Krokhin, O.; Hayglass, K.; Pascoe, E.; Darroch, G.; Rush, D.; Nickerson, P.; Rigatto, C.; Reslerova, M., Mass spectrometry-based proteomic analysis of urine in acute kidney injury following cardiopulmonary bypass: a nested case-control study. Am J Kidney Dis 2009, 53, (4), 584-95.

8. Dwivedi, R. C.; Dhindsa, N.; Krokhin, O. V.; Cortens, J.; Wilkins, J. A.; El-Gabalawy, H. S., The effects of infliximab therapy on the serum proteome of rheumatoid arthritis patients. Arthritis Res Ther 2009, 11, (2), R32.

9. Seifers, D.L.; Martin, T. J.; Harvey, T.L.; Haber, S.; Krokhin, O.; Spicer, V.; Ying, S.; Standing, K.G. Identification of variants of the High Plains virus Infecting Wheat in Kansas. Plant disease 2009, 93 (12), 1265-1274.

10. Seifers, D.L.; Martin, T. J.; Harvey, T.L.; Fellers, J.P.; Stack, J.P.; Ryba-White, M,; Haber, S.; Krokhin, O.; Spicer, V.; Lovat, N.; Yamchuk, A.; Standing, K.G. Triticum mosaic virus: A new virus isolated from wheat in Kansas. Plant disease 2008, 92 (5), 808-817.

11. Qian, Y.; Preston, K.; Krokhin, O.; Mellish, J.; Ens, W., Characterization of wheat gluten proteins by HPLC and MALDI TOF mass spectrometry. J Am Soc Mass Spectrom 2008, 19, (10), 1542-50.

12. Dwivedi, R. C.; Spicer, V.; Harder, M.; Antonovici, M.; Ens, W.; Standing, K. G.; Wilkins, J. A.; Krokhin, O. V., Practical implementation of 2D HPLC scheme with accurate peptide retention prediction in both dimensions for high-throughput bottom-up proteomics. Anal Chem 2008, 80, (18), 7036-42.

13. Azoulay Shemer, T.; Harpaz-Saad, S.; Belausov, E.; Lovat, N.; Krokhin, O.; Spicer, V.; Standing, K. G.; Goldschmidt, E. E.; Eyal, Y., Citrus chlorophyllase dynamics at ethylene-induced fruit color-break: a study of chlorophyllase expression, posttranslational processing kinetics, and in situ intracellular localization. Plant Physiol 2008, 148, (1), 108-18.

14. Weiler, T.; Du, Q.; Krokhin, O.; Ens, W.; Standing, K.; El-Gabalawy, H.; Wilkins, J. A., The identification and characterization of a novel protein, c19orf10, in the synovium. Arthritis Res Ther 2007, 9, (2), R30.

15. Tripet, B.; Cepeniene, D.; Kovacs, J. M.; Mant, C. T.; Krokhin, O. V.; Hodges, R. S., Requirements for prediction of peptide retention time in reversed-phase high-performance liquid chromatography: hydrophilicity/hydrophobicity of side-chains at the N- and C-termini of peptides are dramatically affected by the end-groups and location. J Chromatogr A 2007, 1141, (2), 212-25.

16. Spicer, V.; Yamchuk, A.; Cortens, J.; Sousa, S.; Ens, W.; Standing, K. G.; Wilkins, J. A.; Krokhin, O. V., Sequence-specific retention calculator. A family of peptide retention time prediction algorithms in reversed-phase HPLC: applicability to various chromatographic conditions and columns. Anal Chem 2007, 79, (22), 8762-8.

17. She, Y. M.; Krokhin, O.; Spicer, V.; Loboda, A.; Garland, G.; Ens, W.; Standing, K. G.; Westmore, J. B., Formation of (bn-1 + H2O) ions by collisional activation of MALDI-formed peptide [M + H]+ ions in a QqTOF mass spectrometer. J Am Soc Mass Spectrom 2007, 18, (6), 1024-37.

18. Moore, D. F.; Krokhin, O. V.; Beavis, R. C.; Ries, M.; Robinson, C.; Goldin, E.; Brady, R. O.; Wilkins, J. A.; Schiffmann, R., Proteomics of specific treatment-related alterations in Fabry disease: a strategy to identify biological abnormalities. Proc Natl Acad Sci U S A 2007, 104, (8), 2873-8.

19. Meng, X.; Krokhin, O.; Cheng, K.; Ens, W.; Wilkins, J. A., Characterization of IQGAP1-containing complexes in NK-like cells: evidence for Rac 2 and RACK1 association during homotypic adhesion. J Proteome Res 2007, 6, (2), 744-50.

20. Falzarano, D.; Krokhin, O.; Van Domselaar, G.; Wolf, K.; Seebach, J.; Schnittler, H. J.; Feldmann, H., Ebola sGP--the first viral glycoprotein shown to be C-mannosylated. Virology 2007, 368, (1), 83-90.

21. Snovida, S. I.; Chen, V. C.; Krokhin, O.; Perreault, H., Isolation and identification of sialylated glycopeptides from bovine alpha1-acid glycoprotein by off-line capillary electrophoresis MALDI-TOF mass spectrometry. Anal Chem 2006, 78, (18), 6556-63.

22. Lattova, E.; Kapkova, P.; Krokhin, O.; Perreault, H., Method for investigation of oligosaccharides from glycopeptides: direct determination of glycosylation sites in proteins. Anal Chem 2006, 78, (9), 2977-84.

23. Krokhin, O. V.; Ying, S.; Cortens, J. P.; Ghosh, D.; Spicer, V.; Ens, W.; Standing, K. G.; Beavis, R. C.; Wilkins, J. A., Use of peptide retention time prediction for protein identification by off-line reversed-phase HPLC-MALDI MS/MS. Anal Chem 2006, 78, (17), 6265-9.

24. Krokhin, O. V.; Antonovici, M.; Ens, W.; Wilkins, J. A.; Standing, K. G., Deamidation of -Asn-Gly- sequences during sample preparation for proteomics: Consequences for MALDI and HPLC-MALDI analysis. Anal Chem 2006, 78, (18), 6645-50.

25. Krokhin, O. V., Sequence-specific retention calculator. Algorithm for peptide retention prediction in ion-pair RP-HPLC: application to 300- and 100-A pore size C18 sorbents. Anal Chem 2006, 78, (22), 7785-95.

26. Falzarano, D.; Krokhin, O.; Wahl-Jensen, V.; Seebach, J.; Wolf, K.; Schnittler, H. J.; Feldmann, H., Structure-function analysis of the soluble glycoprotein, sGP, of Ebola virus. Chembiochem 2006, 7, (10), 1605-11.

27. Bykova, N. V.; Rampitsch, C.; Krokhin, O.; Standing, K. G.; Ens, W., Determination and characterization of site-specific N-glycosylation using MALDI-Qq-TOF tandem mass spectrometry: case study with a plant protease. Anal Chem 2006, 78, (4), 1093-103.

28. Schaub, S.; Wilkins, J. A.; Antonovici, M.; Krokhin, O.; Weiler, T.; Rush, D.; Nickerson, P., Proteomic-based identification of cleaved urinary beta2-microglobulin as a potential marker for acute tubular injury in renal allografts. Am J Transplant 2005, 5, (4 Pt 1), 729-38.

29. Lattova, E.; Snovida, S.; Perreault, H.; Krokhin, O., Influence of the labeling group on ionization and fragmentation of carbohydrates in mass spectrometry. J Am Soc Mass Spectrom 2005, 16, (5), 683-96.

30. Krokhin, O. V.; Ens, W.; Standing, K. G., MALDI QqTOF MS combined with off-line HPLC for characterization of protein primary structure and post-translational modifications. J Biomol Tech 2005, 16, (4), 429-40.

31. Hasinoff, B. B.; Wu, X.; Krokhin, O. V.; Ens, W.; Standing, K. G.; Nitiss, J. L.; Sivaram, T.; Giorgianni, A.; Yang, S.; Jiang, Y.; Yalowich, J. C., Biochemical and proteomics approaches to characterize topoisomerase IIalpha cysteines and DNA as targets responsible for cisplatin-induced inhibition of topoisomerase IIalpha. Mol Pharmacol 2005, 67, (3), 937-47.

32. Ethier, M.; Krokhin, O.; Ens, W.; Standing, K. G.; Wilkins, J. A.; Perreault, H., Global and site-specific detection of human integrin alpha 5 beta 1 glycosylation using tandem mass spectrometry and the StrOligo algorithm. Rapid Commun Mass Spectrom 2005, 19, (5), 721-7.

33. Meng, X.; Cheng, K.; Krohkin, O.; Mould, A. P.; Humphries, M. J.; Ens, W.; Standing, K.; Wilkins, J. A., Evidence for the presence of a low-mass beta1 integrin on the cell surface. J Cell Sci 2005, 118, (Pt 17), 4009-16.

34. Lattova, E.; Perreault, H.; Krokhin, O., Matrix-assisted laser desorption/ionization tandem mass spectrometry and post-source decay fragmentation study of phenylhydrazones of N-linked oligosaccharides from ovalbumin. J Am Soc Mass Spectrom 2004, 15, (5), 725-35.

35. Krokhin, O. V.; Craig, R.; Spicer, V.; Ens, W.; Standing, K. G.; Beavis, R. C.; Wilkins, J. A., An improved model for prediction of retention times of tryptic peptides in ion pair reversed-phase HPLC: its application to protein peptide mapping by off-line HPLC-MALDI MS. Mol Cell Proteomics 2004, 3, (9), 908-19.

36. Krokhin, O.; Ens, W.; Standing, K. G.; Wilkins, J.; Perreault, H., Site-specific N-glycosylation analysis: matrix-assisted laser desorption/ionization quadrupole-quadrupole time-of-flight tandem mass spectral signatures for recognition and identification of glycopeptides. Rapid Commun Mass Spectrom 2004, 18, (18), 2020-30.

37. Ghosh, D.; Krokhin, O.; Antonovici, M.; Ens, W.; Standing, K. G.; Beavis, R. C.; Wilkins, J. A., Lectin affinity as an approach to the proteomic analysis of membrane glycoproteins. J Proteome Res 2004, 3, (4), 841-50.


Selected Publications (ion chromatography and capillary electrophoresis):

1. O.V.Krokhin, A.D.Smolenkov, N.V.Svintsova, O.N.Obrezkov, O.A.Shpigun. "Modified silica as a stationary phase for ion chromatography". J.Chromatogr. A. V.706 (1995) P. 93-97.

2. O.V. Krokhin, W. Xu, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. “Simultaneous determination of inorganic anions and equally charged complexes of transition metals with polyaminocarboxylic acids by capillary electrophoresis”. Chemistry Letters. 1996. P. 1095-1096.

3. O.V. Krokhin, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. “Influence of cationic polymers on separation selectivity in kinetic differentiation mode capillary electrophoresis of metal-4-(2-pyridilazo)resorcinolato chelates”. J. Chromatogr. A. V. 772. (1997) P. 339-346.

4. O.V. Krokhin, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. “Use of cationic polymers for the simultaneous determination of inorganic anions and metal-PAR chelates in kinetic differentiation mode capillary electrophoresis”. J. Chromatogr. A. V. 776(2). (1997) P. 329-336.

5. A.V. Pirogov, N.V. Svintsova, O.V. Kuzina, O.V. Krokhin, M.M. Platonov, O.A. Shpigun. "Silicas modified by polyelectrolyte complexes for the ion chromatography of anionic complexes of transition metals". Fresenius J.Anal.Chem. V.361.1998. P.288-293.

6. O.V. Krokhin, A.V. Adamov, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. "Separation selectivity of some ethylenediaminetetraacetic acid and cyclohexane-1,2-diaminetetraacetic acid complexes in column and ion electrokinetic chromatography". J. Chromatogr. A. V. 850 (1-2). (1999) P. 269-276.

7. O.V. Krokhin, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. "The use of N,N"-Bis(hydroxybenzyl)ethylenediamine-N,N"-diacetic acid (HBED) for the determination of 3+ metal ions by capillary electrophoresis". Chemistry Letters. 1999. P. 903-904.

8. A.V. Pirogov, O.V. Krokhin, M.M. Platonov, Ya.I. Deryugina, O.A. Shpigun “Ion-chromatogpaphic selectivity of polyelectrolyte sorbrnts based on some aliphatic and aromatic ionenes” J. Chromatogr. A. V. 884 (2000) P. 31-39.

9. O.V. Krokhin, O.V. Kuzina, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. "Separation selectivity of anionic metal complexes of N,N'-Bis(hydroxybenzyl)ethylenediamine-N,N'-diacetic acid in ion and ion electrokinetic chromatography". J. Chromatogr. A. V. 895 (2000) P. 255-261.

10. O.V. Krokhin, H. Hoshino, O.A. Shpigun, T. Yotsuyanagi. "Potential of Ethylenediaminedi-(o-hydroxyphenylacetic acid) (EDDHA) and N,N'-Bis(hydroxybenzyl)ethylenediamine-N,N'-diacetic acid (HBED) for the determination of metal ions by Capillary Electrophoresis". J. Chromatogr. A. V. 890 (2000) P. 363-369.

11. V.L. Shapovalov, T.I. Rokitskaya, E.A.Kotova, O.V.Krokhin, Yu.N. Antonenko. “Effect of Fluoride Anions on Gramicidin Photoinactivation Sensitized by Sulfonated Aluminium Phtalocyanines”. Photochemistry and Photobiology. V. 47 (1). (2001) P. 1-7.


AWARDS:

1992: J. Soros Fund scholarship for young Russian scientists

1994: Scholarship of Russian Federation President for young Russian scientists

1998: Moscow State University Prize for young scientists

2006: Brockhouse Canada Prize for interdisciplinary research in Science and Engineering

PATENTS:

Krokhin, O.V.; Spicer, V., A composition for use as a peptide retention standard and a method of predicting hydrophobicity in liquid chromatography. U.S. Provisional patent application No. 61/118,546 (2008).

Krokhin, O.V.; Spicer, V., Methods for determining the retention of peptides in reverse phase chromatography using linear solvent strength theory. U.S. Provisional patent application No. 61/318,037 (2010).

Krokhin, O.V.; Spicer, V., Methods and systems for analysis of peptide sample streams using tandem mass spectroscopy. U.S. Provisional patent application No. 61/346,678 (2010).

J.A. Wilkins, H. El-Gabalawy, O. Krokhin, K. Dasuri, Q. Du, , W. Ens, Characterization of c19orf10, a novel synovial protein' US patent application 11/746,439 (2007).